Radioimmunoprecipitation assay definition. 1. 2. of an ore or drug: His find required an assay to determine its value. Jun 9, 2025 ยท The meaning of RADIOIMMUNOPRECIPITATION is immunoprecipitation using antibodies or antigens labeled with a radioisotope. Introduction Radioimmunoprecipitation Assay Buffer (RIPA) is used to lyse cells and tissues for use in radioimmunoprecipitation, protein assays, protein purification and other analytical procedures. Radioimmunoprecipitation Assay (RIPA) is a powerful tool used to measure and quantify the amount of specific proteins in biological samples. AI generated definition based on: AIDS and Other Manifestations of HIV Infection (Fourth Edition), 2004 What does radioimmunoprecipitation assay mean? Information and translations of radioimmunoprecipitation assay in the most comprehensive dictionary definitions resource on the web. Lab medicine The quantification of a substance of interest by a specific chemical, enzymatic, immunologic or radioimmune-mediated reaction. g. an analysis of a substance, esp. It is based on the concept of antigen-antibody binding and utilizes an antiserum containing antibodies specific to the antigen of interest. This protocol outlines the preparation of RIPA . Viral-infected cell cultures are radioactively labeled and lysed to yield radiolabeled antigen fragments. Define radioimmunoprecipitation assay. Due to its ionic detergent composition, RIPA can disrupt nuclear membranes and solubilize cytoplasmic proteins, resulting in a high protein yield. Radioimmunoprecipitation (RIPA) is defined as a qualitative test used for the confirmation of viral antigens, involving the use of radiolabeled antigen fragments obtained from the lysis of virus-infected cell cultures. Radioimmunoprecipitation assay (RIPA) is used routinely for the detection of viral proteins, characterization of monoclonal and polyclonal antibody preparations, and determination of the specificity of the immune response to various pathogens (1-3). (RIPA) is the term used to describe the qualitative assay used as a confirmatory procedure for some antibodies to viral antigens. Definition of 'radioimmunoprecipitation' radioimmunoprecipitation noun biochemistry the separation of an antigen from a solution by the formation of a large complex with its specific antibody labelled with a radioisotope Radioimmunoprecipitation is defined as a complex assay technique that involves the use of radiolabeled lysates reacted with test serum to isolate immune complexes, allowing for the detection of specific viral antibodies, such as gp120 and gp160, through electrophoresis and film exposure. A good strategy is first to lyse cells using a relatively strong buffer such as radioimmunoprecipitation assay buffer (commonly referred to as RIPA buffer) containing the ionic detergents SDS and sodium deoxycholate (DOC). AI generated definition based on: Blood Banking and Transfusion Medicine (Second Edition), 2007 What does radioimmunoprecipitation assay mean? Information and translations of radioimmunoprecipitation assay in the most comprehensive dictionary definitions resource on the web. Not to be confused with: essay – try; subject to a Radioimmunoprecipitation is a complex and arduous technique used to supplement antibody detection procedures, particularly in distinguishing between infections such as HTLV-1 and HTLV-2. To examine or analyze. Qualitative or quantitative analysis of a substance such as a drug. A test of the biologic potency of something (e. radioimmunoprecipitation assay synonyms, radioimmunoprecipitation assay pronunciation, radioimmunoprecipitation assay translation, English dictionary definition of radioimmunoprecipitation assay. , medicine). sr ai7j fwn4 zptx 6em5g 2km lwuz0b 3at7 w8 gn92