Protein concentration kit. Purification is based on spin column chromatography using .



Protein concentration kit. The protein concentration of each sample was determined using the bicinchoninic acid (BCA) assay (Sigma-Aldrich, USA). Achieve sensitive detection of protein, and low protein-to-protein variation, with the Qubit Protein and Protein BR Assay Kits for fluorescence-based protein quantitation assays. The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. Regardless of the sample type you have, it is strongly recommended that you sub-aliquot all samples after preparation to minimize protein degradation from multiple freeze-thaw cycles. The most common protein assay reagents involve The QubitTM Protein Assay Kits make protein quantitation easy and accurate. BCA protein assay in a 96 well plate The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Fast and ready-to-use. Carefully separate the supernatant and transfer into a new tube. The purified urine proteins are both concentrated and The kit has been optimized for determining total protein content in low protein concentration samples (0. 2-D Quant Kit is designed to accurately determine protein concentrations in samples for electrophoresis. Explore our Bradford assays for protein quantification. http://www. Endotoxin-Free Proteins. All work should be performed inside a BSC. Bicinchoninic (BCA) Acid Protein Assay provides accurate determination of protein concentration with most sample types encountered in protein research. - Detergent compatible (up to 5%) The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. The ProteoSpinTM Urine Protein Concentration Micro Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from small volumes of urine. In acidic conditions, the dye exists in a reddish-brown cationic form (maximum absorbance at 470 nm). Reduction, Alkylation and Acetone Product Information The Amicon® Pro Affinity Concentration Kit - Protein G (buffers, resin, devices) was optimized for maximal binding and elution of Ig species. Product Insert The ProteoSpinTM Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Then, Cu+ interacts with the unique BCA Reagent A (containing BCA) to preform a sensitive color reaction, forming coordination complexs. 4. This water-soluble complex exhibits a strong absorbance at 480 nm that is very linear with increasing protein concentrations over a broad working The protocols defined in this User Guide apply to all of the LabChip instrument products running the Protein Express Assay. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Determine the protein concentration of the supernatant using established methods such as the BCA Protein Assay Kit (e. Proteins are quantitatively precipitated while interfering substances are left in solution. The assay can measure accurately protein concentrations ranging from 0. Accordingly, the Thermo Scientific Modified Lowry Protein Assay Kit is ideal for loyal Lowry method users who would like the increased convenience of a stable, pre-formulated product. Quantify total protein concentration using a total protein assay. Description Pierce GST Protein Interaction Pull-Down Kit, contains sufficient materials for conducting 25 pull-down assays using a GST-tagged fusion protein as the bait Get fast, accurate protein concentration and purity analysis for a wide range of proteins with the Protein Express assay on the LabChip GXII Touch system. Addressing overlapping concentration ranges, the two kits together are accurate for initial sample concentrations from 12 Protein quantification is a crucial step in many biological research and industrial processes. 5, 0. For more information on the theory and benefits of Revvity’s HTRF technology, including protein-protein interaction assays, please visit our website at www. Probe 2 will be used for the Total Protein assay. Introduction and Principle The Bio-Rad DC Protein Assay is a colorimetric assay for protein concen-tration following detergent solubilization. Quantify protein quickly and accurately with two unique Qubit protein quantification kits: the Qubit Protein Assay, which quantifies 12. Section 1 Introduction The Bio-Rad Protein Assay, based on the method of Bradford, is a simple and accurate procedure for determining concentration of solubi-lized protein. Simply dilute the reagent using the bufer provided, add your sample (any volume from 1–20 μL is acceptable), then read the concentration using the QubitTM Fluorometer. The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Bicinchoninic (BCA) Acid Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total protein concentration compared to a protein standard. The assay is highly selective for protein. 96-Microplate Procedure (1) Prepare the Standard Reagent. 6. 5-30 µg. Introduction The Pierce BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. Protein solutions as dilute as 1 ng/mL can be quantitatively concentrated into a small volume. When BQC Protein Concentration Assay Kit ensures the highest protein recovery and is adapted for higher and lower initial concentrations. The reaction is similar to the well-documented Lowry assay, but has been modified to save time. The ProteoSpin TM Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved. The BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure total protein concentration compared to a protein standard. The QuantiPro™ BCA Assay Kit is used to determine protein concentrations in the range of 0. Proteospin Urine Protein Concentration Kit For the rapid and efficient concentration of total proteins from urine samples Simultaneous concentration, desalting and buffer exchange of total urinary proteins No . Compatible with a broad range of reagents. The Protein Quantification Assay is a convenient and reliable kit for the determination of protein concentration in samples typically used for SDS-PAGE (sodium dodecylsulfate polyacrylamide gel electrophoresis). There are a variety of protein quantitation methods including UV absorbance assays, reagent-based assays, and immunoassay technologies. If these reagents are inevitable, FineTest product- Bradford Protein Assay kit is available (K002). INTRODUCTION Quantitation of nucleic acids (DNA/RNA) and proteins are common measurements in basic science and clinical research. These guidelines are intended to be an educational resource for preparing commonly tested samples for use in ELISA assays. With any protein assay, it is best to use an immunoglobulin reference standard, such as our Bovine Gamma Globulin Standard (BGG), instead of the usual bovine serum albumin (BSA). The protein standard series should cover the range of concentration between 125 and 1000 μg/mL. Pierce™ Modified Lowry Protein Assay Kit. Aug 13, 2020 · 1. Besides, absorbance of protein at 238nm is relevant to the number of peptide bond. The DC protein assay requires only a single 15 minute incubation, and absorbance is stable for at least 2 hours. The Data Reference consists of the Logbook Reference number and Protein Quantification Kit (BCA Assay) Protein Quantification Kit (BCA Assay) Views (16491) Publications (92) Catalog no (KTD3001) Datasheet Print Share Email Share 1 / Total 1 BCA Protein Assay determines protein yields after cell lysis, affinity column fractions, purified protein samples, and monitors contaminants. 05 to 0. Each standard and sample should have its own well (see layout below). Each of these protein quantitation technologies has unique benefits and the suitability of the assay depends on the sample type and/or volume The concentration listed refers to the actual concentration in the protein sample. The prediluted standards are conveniently packaged in The signal produced from this reaction is three- to four-fold more sensitive than the Thermo ScientificTM Micro BCATM Protein Assay Kit for peptide analysis. We provide gentle, membrane-based Protein Concentration options to concentrate proteins without compromising yield, even when labile or low in abundance. 6 mg/ml and from 0. MilliporeSigma | Life Science | Industrial & Lab Chemicals Amicon® Pro Affinity Concentration Kit Protein A with 3kDa Amicon® Ultra-0. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Dilutions of 500× for Naja ashei (Na) and 100× for Agkistrodon contortrix (Ac) were used to measure protein concentration in venom. The measurement can be performed without high instrumental expense. . Use this Dye Reagent Concentrate to refill the Bio-Rad Protein Assay, a simple, accurate method for total protein measurement. The Micro BCA Protein Assay Kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature (60°C, Test Tube Procedure only). The Novagen ® BCA Protein Assay Kit canbe used to determine protein concentrationin the range of 20-2000 µg/ml in either astandard assay or microassay configuration. Centrifuge lysate at 16,000 × g for 10 minutes at 4°C. It provides increased linearity and half the protein-to-protein variability of other commercial Bradford assay formulations. 45 μm polyvinylidene difluoride membranes (Millipore, Massachusetts, USA). A series of dilutions of known concentration are prepared from the protein and assayed alongside the unknown(s) before the concentration of each unknown is determined based on the standard curve. The migration time relates to the size of the proteins: larger proteins move slower due to the sieving effect of the gel inside the chip channels. The procedure is easy to perform and does not rely upon ultracentrifugation. This kit contains ready-to-use Coomassie Brilliant Blue G solution and BSA solution as a protein standard solution, and is suitable for a microplate assay. The kits include concentrated assay reagent, dilution bufer, and prediluted BSA standards. Because the color response with Coomassie is non-linear with increasing protein concentration, a standard curve must be completed with each assay. One major Explore our Bradford assays for protein quantification. The prediluted standards are conveniently packaged in Materials 96-well microplate BCA Reagent A (component of the Pierce BCA Protein Assay Kit) BCA Reagent B ( (component of the Pierce BCA Protein Assay Kit) Protein standards Unknown protein samples Procedure Add 10uL of each standard and your GFP elution “E” into a microplate well in duplicate. While the traditional BCA assay produces a purple reaction product, the PierceTM Rapid Gold BCA Protein Assay Kit produces an orange-gold-colored reaction product, which is formed by the chelation of 2 molecules of the chelator with 1 cuprous ion. Accurate measurement of protein concentration is essential for various applications, such as studying protein-protein interactions, assessing enzyme activity, and determining protein concentration for downstream analyses. Dilutions are Each of the vials of Alexa Fluor® 488 TFP ester supplied in the kit is sufficient for labeling one sample (20–100 μg at a concentration of 1 mg/mL) of protein that has a molecular weight between ~12 and ~150 kDa. Learn comprehensive protocols for basic Sandwich ELISA, Neurobiology ELISA, Phospho ELISA, as well as protocols for PeproTech Sandwich TMB ELISA and Sandwich ABTS ELISA Development kits. The Protein (BCA) test kit is an easy and fast way to analyze the protein concentration in your sample. 1 The properties of DNase I can be modified by divalent ions. Using standard procedure, the assay is used with samples Product description The InvitrogenTM QubitTM Protein BR Assay Kit provides a quick and accurate method to quantitate protein samples over a broad range of concentrations on the QubitTM 4 Fluorometer. The protein concentrates can be used for several applications, including Western blot, SDS-PAGE, or 2D-gels. 8. A series of dilutions of known concentration are prepared from the protein and assayed alongside the unknowns before the concentration of each unknown is determined based on the standard Oct 2, 2016 · 碧云天生产的Bradford蛋白浓度测定试剂盒 (Bradford Protein Assay Kit)是根据最常用的两种蛋白浓度检测方法之一 Precipitation is a protein isolation procedure, which allows physical separation of the protein pellet from the aqueous solution. 7. Learn about the different methods for performing an ELISA assay for protein quantitation, including assay design strategies and reagents. The high binding capacity of the Protein A matrix also provides a rapid and scalable means for depletion of Igs from serum or plasma allowing detection and analysis of low abundant proteins present in The Pierce Top 12 Abundant Protein Depletion Spin Columns remove > 95% of these 12 high-abundant proteins from 10μL of serum or plasma samples and enable the identification and quantitation of low-abundant proteins in samples by mass spectrometry (MS). 00 For rapid and efficient endotoxin removal from proteins and peptides Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution. Hence, it becomes obligatory to measure protein concentration accurately to The staining reaction completes within 1 minute and the color is stable for 30 minutes. Higher protein concentration samples are precipitated much more efficiently compared to those with lower protein concentrations. com +1 7869480594 The Bradford Protein Assay Kit is developed according to the Bradford method and is one of the commonly used methods for protein quantification, which can achieve fast, stable and highly sensitive protein concentration determination with good linearity of detection data. Hence, it becomes obligatory to measure protein concentration accurately to 1. Since I’m working with highly concentrated protein would people recommend I dilute it (and by how much, so far I’ve added 150 uL urea) so I can repeat a BCA assay, and figure out the concentration of my protein? I’m trying to get it within the range of the BCA standards (using Pierce BCA assay kit from thermo) BCA Protein Assay kit is intolerant with reducing or metal chelating reagents. 05% sodium azide How to use UV-vis to determine protein concentration? Protein concentration can be estimated by measuring the UV absorbance at 280 nm; proteins show a strong peak here due to absorbance from Tryptophan and Tyrosine residues (commonly referred to as A280). The Qubit Protein Assay Kits are designed to make protein quantification easy while providing accurate quantification. Protein samples and protein standards are processed in the same manner by mixing them with assay reagent and using a spectrophotometer to measure the absorbances. NOTE: The Bradford assay method requires two separate regression curves for assaying protein concentration, from 0. The dynamic range for this assay is 0. As in a Bradford Assay The Protein (Biuret) test kit is an easy and fast way to analyze the protein concentration in your sample. The analyses were performed in Each of the vials of Alexa Fluor® 488 TFP ester supplied in the kit is sufficient for labeling one sample (20–100 μg at a concentration of 1 mg/mL) of protein that has a molecular weight between ~12 and ~150 kDa. Choose the Bio-Rad Protein Assay for simple, accurate measurement of total protein. This kit allows simultaneous detection of up to 96 samples or standards and requires only 9 μl of protein sample, making it suitable when limited amounts of sample are available The Quick Start™ Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. The two key parameters for protein sizing and quantitation with the Agilent 2100 Bioanalyzer are migration time and fluorescence intensity. The fold-change and concentration of PPP from the XCELL PC filter is more efficient than the competition and captures more key anti-inflammatory cytokines than others on the market. 25–5 KIT SPECIFICATIONS EFFECTIVE CONCENTRATION OF PROTEIN SAMPLES WITH MAXIMUM RECOVERY Figure 5. Bio-Rad Protein Assay Kit The simplicity, sensitivity, and color of the Bio-Rad protein assay make it a great vehicle for integrating biology, math, experimental design, and technique ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit SKU 22800 | Mini | 25 Preps USD285. 5 mg/mL), similar to Lowry protein assay, Bradford protein assay or biuret reagent. Dec 14, 2017 · CALBIOCHEM The Cytosol/Mitochondria Fractionation Kit is designed for extraction of cellular proteomes from mammalian cells and deliver two distinct proteins fractions: a mitochondrial fraction and a cytosolic fraction. Since the curve flattens at high concentrations of dye, the amount of protein in the A number of assays are available for the determination of protein concentration, each with a set of advantages. The protein reduces Cu2+ to Cu+ under alkaline conditions. Bradford Assay Standards The assay uses standards to both quantify the amount of protein in samples and to subtract any background due to interfering substances that can shift the ratios between the three forms of the dye. The ProteoSpinTM Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is also designed for the rapid removal of endotoxins from up to 200 g of previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Fluorescence intensity, in turn, is related to the concentration of these proteins, which are indirectly Antibody purification kits Abcam's antibody concentration and clean-up kits are designed to streamline the process of concentrating antibodies and proteins, ensuring their compatibility with various conjugation kits. - Find MSDS or SDS, a COA, data sheets and more information. We recommend using Bio-RadTM’s DC Protein Assay Kit. Introduction The Quant-iTTM Protein Assay Kit makes protein quantification easy and accurate. Jun 18, 2025 · Absorbance is proportionate to protein content. 2100 Protein-Concentrate Kit (Micro) is suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays & other applications. The BCA protein concentration determination kit can be used for colorimetric assay and microplate assay. This colorimetric assay based on the Bradford method is compatible with many common lab reagents. The kit is compatible with qEV Isolation, offering a comprehensive solution for Extracellular Vesicle sample processing. Jun 27, 2018 · Your target total protein concentration of the homogenate should be at least 1,000 µg/mL, but 2,000 µg/mL or more would be better. com. Available in 530 mL The Chemicon Protein-Concentrate kit uses a proprietary reagent Universal Protein Precipitation Agent (UPPA). Sep 18, 2025 · Total protein kit, micro provides a simple, rapid, and highly sensitive method to determine the concentration of proteins in solution. The Thermo Scientific Pierce Coomassie Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a. In addition, the Pierce 660 nm Protein Assay is compatible with high concentrations of most commonly used reagents, such as detergents and reducing agents. 5 μg/mL to 5 mg/mL of protein, and the Qubit Protein BR Assay Kit, which quantifies 0. 10410 Corporate Dr, STE12 Sugar Land, TX 77478, USA ELKbio@ELKbiotech. The ProteoExtract Protein Precipitation Kit is designed for the concentration and clean-up of proteins from aqueous samples. The sample was processed as per the provided protocol Note: All specifications and protocol instructions presented below are specific to the pedestal mode for NanoDropTM 2000/2000c instruments. The protein is mixed with the dye and heated; as it unfolds or melts BCA Protein Assay Kit The BCA protein assay is a simple and reliable protein quantification method. The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s). The former requires a large amount (100 μL) of protein sample, The ratio of protein sample to BCA working solution is 1:20 (v/v), which reduces the impact of interfering substances. The assay, which relies on BCA, copper, and alkaline conditions, leads to the formation of complexes between copper ions and polypeptides. Bradford assay reagents, BCA assay kits, bovine serum albumin (BSA) standards, and other spectrophotometer and microplate protein assay products used to precisely determine and measure total protein concentration following cell lysis, labeling or purification. This User Guide reviews the general principles and steps in developing a protein-protein interaction assay using HTRF PPi reagents from Revvity. Overview Abcam's BCA Protein Quantification Kit provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution. 1 mg/ml to 50 mg/ml depending upon the progress of nephritis, various dilutions of individual samples are required. The result is an extremely sensitive colorimetric protein assay in a test tube or microplate assay format. Inhibitor binding real-time melt experiment using the Protein Thermal Shift dye kit and any real-time PCR system will yield a fluorescence profile specific to a protein and its environment (buffer or ligands). After precipitation, the precipitate is suspended in a small volume of The Bradford protein assay is a simple colorimetric assay for measuring total protein concentration using a dye-binding method based on the Bradford assay. 5 μg/mL to 1. Our ready-to-use Bradford reagents are quick and easy to use for protein concentration determination. The objective is to select a method that requires the least manipulation or The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up, and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Protein fractions are suitable for many downstream applications including 1D and 2D gel The Bradford Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. The assay is accurate for sample concentrations from 100 μg/mL to 20 mg Bradford assay reagents, BCA assay kits, bovine serum albumin (BSA) standards, and other spectrophotometer and microplate protein assay products used to precisely determine and measure total protein concentration following cell lysis, labeling or purification. The kit also contains a high quality peptide digest reference standard to generate linear standard curves and calibration controls. A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method. After plotting in excel you will get straight line, click on this graph and tick Kit Contents: Pierce 660nm Protein Assay Reagent, 450mL Pre-diluted Protein Assay Standards: Bovine Serum Albumin (BSA) Set, 7 × 3. Typical methods for the colorimetric determination of protein concentration in solution include the Coomassie blue G-250 dye–binding assay [1], the biuret method [2], the Lowry method [3], the bicinchoninic acid (BCA) assay [4], and the colloidal gold protein assay [5]. Because the protein concentration in rat urine can vary from 0. Product description The Thermo ScientificTM PierceTM BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. Prepare a standard curve by plotting the average Blank-corrected 750 nm values for each BSA standard vs. Transfer the supernatant to a new microcentrifuge tube. Endotoxins were removed from 100 μg protein samples in triplicate using the ProteoSpinTM Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reaction) with the highly Introduction Understanding the complex, coordinated protein interactions that sustain life may enable us to unlock mysteries of disease mechanisms and thereby discover treatments. Please refer to the reagent manufacturer for additional guidance when utilizing the cuvette mode of the NanoDrop 2000c. After precipitation, the precipitate is suspended in a small volume of BCA Assay Kits BCA assay kits contain the reagents needed to measure total protein concentrations using the bicinchoninic acid (BCA) method. 5, and 2. Qubit protein assays require only 10–15 minutes of room temperature incubation, eliminating long incubation periods or exposure to elevated temperatures. 25, 0. protein concentrates can be used for several applications such as Western blot, SDS-PAGE or 2D-gels. The Bicinchoninic Acid (BCA) Protein Assay is a widely used method for protein Protein concentration determination is often essential before processing protein samples for isolation, separation and analysis by chromatographic, electrophoretic and immunochemical techniques. The kits include concentrated assay reagent, dilution buffer, and prediluted BSA standards. Both assays are compatible with many common contaminants, reducing reagents, salts, and nucleic In this webinar, we will discuss the principles behind the most popular protein concentration assays, how to achieve the highest quality results with your sample, and the importance of using the proper standards, protein preparation and others. The ProteoSpin&trade; Total Protein Concentration Micro Kit provides a fast and a simple procedure for the concentration of protein samples many fold in a convenient spin column format. The endotoxin-spiked BSA was then cleaned, in triplicate, using the ProteoSpinTM Total Protein Concentration Amicon Pro Affinity Concentration Kit Protein A - A Centrifugal Tool for purifying & concentrating Antibodies. Protein quantitation and protein assays are critical for accurately determining the protein concentration in a sample. The reagent combines the useful properties of low protein to protein variance (See Fig 1) an d a wide protein concentration for a robust assay. Jul 1, 2025 · Efficiently concentrate intact Extracellular Vesicles (EVs) for critical downstream analyses like protein and RNA studies. One of the most common is the bicinchoninic acid (BCA) assay, which utilizes copper reduction from the proteins’ peptide bonds. Protocols linked below are to be used as a guide only and may require optimization for your experiment. Generally, the following substances should be avoided for the given protein assay method: BCA and Micro BCA Assays: reducing agents and chelators, and strong acids and bases BCA Reducing Agent Compatible Assay: chelators 660 nm Assay: ionic detergents Pierce Bradford Protein Assay Kit and Pierce Bradford Plus Protein Assay Kit Pierce™ 660nm Protein Assay Reagent. An Ø denotes compounds that were not compatible at the lowest concentrations tested. Equal amounts of proteins (20 μg) were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 0. its concentration in μg/mL. UPCKTM Urine Protein Concentration Kit has been especially optimized for proteomic studies of urine proteins. 6 billion platelets. The generated Cu2+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong The CBQCA Plus Protein Quantitation Kit is an updated version of the CBQCA Protein Quantitation Kit (Catalog No. The choice among available protein assays usually is based upon the compatibility of the method with the samples to be assayed. The Bio-Rad protein and Quick Start™ Bradford protein assays are recommended for classroom use. The assay is accurate for initial sample Jun 28, 2022 · ELK Biotechnology CO. g. It involves the addition of an acidic dye to protein solution, and subsequent measurement at 595 nm with a spectrophotometer or microplate reader. Concentrated EVs are particularly suited to protein and RNA The Quick Start Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. (5), X real and X pred represent the protein concentration estimated from the molecular mass of proteins and the predicted protein concentration (determined by the BCA assay), respectively. Such kits may utilize centrifugal concentrators, typically disposable spin columns that require only benchtop centrifuges. Product description The Thermo ScientificTM PierceTM Bradford Plus Protein Assay Kit is a quick and ready-to-use Coomassie-binding, colorimetric method for total protein quantitation. 5 to 1. Nevertheless, in spite that the general principle behind this methodology is known, there are still unanswered questions re … May 29, 2025 · Protein quantification is an indispensable step in a wide range of biochemical and molecular biology applications. Simply dilute the reagent 1:200, load 200 μL into the wells of a microplate, add 1–20 μL sample volumes, mix, then read the fluorescence. May 29, 2025 · Protein quantification is an indispensable step in a wide range of biochemical and molecular biology applications. BCA forms a This kit concentrates dilute solutions of proteins from small volumes of urine using a rapid and convenient spin column. 9% saline and 0. The DC (detergent compatible) protein assay is a colorimetric assay for protein concentration following detergent solubilization. Maxi Kit The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Samples containing 100 μg of BSA were spiked with endotoxins (0. Endotoxins Apr 16, 2020 · Prepare a known protein concentration series by diluting a stock bovine serum albumin (BSA) standard (or other protein standard) in the same diluent as the protein sample whose concentration is to be determined. Material The Precision Red Advanced Protein Assay Reagent is designed to optimize the speed and accuracy of protein measurement. The color density that develops in the 2-D Quant Kit procedure is inversely related to the protein concentration, with a linear response to protein in the range of 0–50 µg and a volume range of 1 Nov 21, 2012 · using protein assay e. In the range of 0. Tissue Lysates – Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. BCA protein assay kit ab102536 is a simple, rapid, kit for determining the concentration of proteins in solution, within the range of 0. Use the standard curve to determine the protein concentration for each unknown sample. The Thermo Scientific Pierce Micro BCA Protein Assay Kit is a special 3-component version of our popular BCA Reagents, optimized to measure (A562nm) total protein concentration of dilute protein solut. Available in 750 mL Protein determination by the bca method Product Description Cayman’s Protein Determination (BCA) Kit is a quantitative assay that can be used with various buffers, water, plasma, serum, urine, and cell lysates. bio-rad. 2 In the presence of Try the RC DC protein assay for rapid protein determination in the presence of reducing agents and/or detergents. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes. Use this simple colorimetric protein assay to measure total protein concentration. Convenient, standard concentration data points are 125, 250, 500, 750 and 1000 μg/mL Nov 1, 2020 · Amongst the available methodologies for protein determination, the bicinchoninic acid (BCA) assay highlights for its simplicity, sensitivity, repeatability and reproducibility. The measurement can be performed without high-cost instrumentation. 5 Device Amicon Pro Affinity Concentration Kit Protein A - A Centrifugal Tool for purifying & concentrating Antibodies. The Chemicon Protein-Concentrate kit uses a proprietary reagent Universal Protein Precipitation Agent (UPPA). We have found this method consistent and seems to work for majority of tissues. As there are many different methodologies available to scientists today, it can be Dear Arooje Nasir You have to use increasing concentration of known protein (BSA) to prepare a standard curve. G-Biosciences Protein concentration kit ensures the highest protein recovery and is adapted for higher and lower initial concentrations. UPCKTM Urine Protein Concentration Kit is a polymeric silica-based protein enrichment matrix designed as an alternative to ultra filtration and solvent precipitation. It gradually lyses cells, separates the intact nuclei from the The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Since there is no molecular weight Bradford Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent for measurement of total protein concentration compared to a protein standard. Each protein assay method has its particular sensitivities. Estimation of macromolecule concentration is necessary in protein purification, electrophoresis, biochemistry, cell biology, molecular biology and clinical research applications. Our PRP kits and protein concentrate systems, including innovations in platelet-rich fibrin (PRF) treatments, are transforming patient care. Revvity. BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates. Flexible assay formats: Use the standard assay for protein concentrations between 200 and 1,400 µg/ml (20–140 µg total protein) Use the low-concentration assay for protein concentrations < 25 µg/ml (1–20 µg The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. The Pierce Bradford Plus Protein Assay Kit is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent used to quickly measure total protein concentration compared to a protein standard. Selection of the Protein Assay When it is necessary to determine the total protein concentration in a sample, one of the first issues to consider is the selection of a protein assay method. Oct 27, 2016 · BCA蛋白浓度测定试剂盒 (BCA Protein Assay Kit)是根据目前世界上最常用的两种蛋白浓度检测方法之一BCA法研制而成，实现了蛋白浓度测定的简单、高稳定性、高灵敏度和高兼容性。 Accordingly, protein concentrations generally are determined and reported with reference to standards of a common protein such as bovine serum albumin (BSA). Concentration Kits Concentration kits are available to readily prepare protein and nucleic acid samples. 0 mg/ml). Pierce™ Bradford Protein Assay Kit. The LabChip GXII Touch is used for general visual reference in most examples in this guide. The reaction is similar to the well-doc-umented Lowry1 assay, but with the following improvements: The reaction reaches 90% of its maximum color development within 15 minutes thereby saving valuable time, and the color changes not more than 5% in 1 hour The Quick Start Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. When using ELISA kits, please refer to the detailed protocol in the product sheet provided with the kit. A protein assay can be set up to measure the concentration of the unknown protein sample (mg/ml), or it can be set up to determine the total amount of protein in the unknown protein sample (mg). 5-30 μg/mL. The Protein Thermal Shift dye does not fluoresce in aqueous solutions but fluoresces in nonpolar environments. Calculating concentration of target protein in the sample To determine the concentration of each sample, first find the absorbance value on the y-axis and extend a horizontal line to the standard curve. Find out more. Assay immediately or aliquot and store at ≤ -20 °C. Introduction The Thermo Scientific Pierce Microplate BCA Protein Assay Kit – Reducing Agent Compatible enables quantitation of total protein in samples while minimizing interference from disulfide reducing agents. This modification of the well-known Bradford method greatly reduces the tendency of Coomassie reagents to give nonlinear response curves by a formulation that substantially improves linearity for a defined range Total protein kit, micro provides a simple, rapid, and highly sensitive method to determine the concentration of proteins in solution. Aug 8, 2021 · This will be the final protein concentration or the whole concentration as you have mentioned and will correspond to the protein concentration of the stock. ” 8. A known BSA standard curve is used to confirm protein concentrations and to calculate the unknown sample’s protein concentration. This is achieved by replacing the KCN component with mandelonitrile, an alternative nitrile (CN) compound prepared in dimethyl sulfoxide (DMSO BCA Protein Assay Kit The BCA protein assay is a simple and reliable protein quantification method. Pierce protein concentrators are disposable ultrafiltration centrifugal devices used to concentrate, desalt, and buffer exchange biological samples, such as proteins and nucleic acids, readying them for downstream applications. Simply dilute the reagent using the buffer provided, add your sample (any volume from 1–20 μL is acceptable), then read the concentration using the Qubit® Fluorometer. Smith at the Pierce Chemical Company, [1] is a biochemical assay for determining the total concentration of protein in a solution (0. Based on Beer-Lambert law, absorbance A is linear to extinction coefficient, protein concentration C and optical path length I: A=E•C•l. Conjugates produced with the bright and photostable Alexa Fluor 647 dye have absorption and fluores- cence emission maxima of approximately 650 nm and 668 nm, respectively (Figure 1). 5. Quantitation of nucleic acids or proteins can be separated into two groups: direct Introduction Thermo ScientificTM DNase I is commonly used to degrade unwanted single- and double-stranded DNA into 5 ́ phosphodinucleotide and oligonucleotide fragments. Protein Concentration Kits, Protein Extraction Kits Animal Nuclear and Cytoplasmic Extraction Kit (Multi-application Type)-Yamaybio Description This product enables the convenient and efficient separation of nuclear proteins and cytoplasmic proteins from animal cells or tissues. The ELISA is performed and the concentrations of protein determined from the standard curve. Thus, protein concentration can be calculated via measuring absorbance. The Thermo Scientific Pierce 660 nm Protein Assay is a ready-to-use, detergent- and reducing agent-compatible assay reagent to quickly measure (A660 nm) total protein concentration compared to a prote. Pierce Protein Reagent Assay BCA Kit. Select membrane tubing, cassettes, spin columns, flasks, and more. Protein precipitation is not effected by the presence of detergents, chaotropic, or other common laboratory agents. If precise quantitation of an unknown protein is required, it is advisable to select a protein Pierce Biotechnology 3747 N. A Complete System for All Your Needs Protein Concentrate contains beneficial proteins, like A2M, IL1-ra, sTNF, PDGF-BB, and others while adding up to an additional 1. coli. Product Insert Urine protein analysis can be used for the identification of potential biomarkers in urine, and to diagnose and/or monitor renal and other diseases. Qubit Protein BR Assay—fast, accurate protein quantitation Protein quantitation is an integral part of many protein biology workflows and a necessary step before commonly used techniques such as protein electrophoresis, western blotting, mass spectrometry, and immunoassays. This kit simultaneously removes salts while concentrating dilute urine proteins ready for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry and protein microarrays. 5 mg/ml. Upon dissolving the protein pellet, one may adjust the protein concentration of the sample and select a suitable buffer appropriate for analysis, such as electrophoresis, protein assay, and MALDI-MS analysis. 1 to 20 mg/mL of protein. Protein quantitation is most commonly performed using colorimetric assays. 125, 0. The assay is reproducible, rapid and more linear than the Bradford Assay. 4 days ago · The Amplite® Colorimetric Bradford Protein Quantitation Assay Kit is a colorimetric assay that quantifies protein concentration by measuring the absorbance shift of Coomassie Brilliant Blue G-250 dye from brown to blue. Easily adaptable from low to high concentration for rapid 96-well assays. The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. 500 tube, 5,000 microplate assays. Mar 5, 2012 · Protein concentration was determined using the Pierce BCA Protein Assay Kit. The kit includes Protein BR Assay Reagent, Protein BR Assay Bufer, Standard 1 (0 mg/mL BSA) and Standard 2 (10 mg/mL BSA). It has the characteristics of high sensitivity, light background and significant color difference, and the measured of protein in low concentration can be intuitive and convenient. The BCA Protein Assay Kit is based on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+ by cysteine, cystine, tryptophan, tyrosine, and peptide bonds. These reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The total protein Learn about sample dialysis devices, desalting columns, and concentrators for biological sample prep and buffer exchange. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reaction) with the highly The qEV Concentration Kit captures intact EVs isolated using any of the qEV Columns producing elution volumes ranging from 600 μL to 20 mL. Available in 950 mL BCA Protein Assay - Reducing Agent Compatible – reducing agent–compatible version of our BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants. Protein-Concentrate Kit (Med) - Find MSDS or SDS, a COA, data sheets and more information. ,Ltd. To further demonstrate the ability of the ProteoSpinTM Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit to concentrate protein samples, 50 μg of BSA was spiked into 2 mL of a 50 mM sodium acetate solution (pH 4. Buy from Sigma Aldrich. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a MilliporeSigma® Amicon® Pro Affinity Concentration Kit Protein G Brand: Millipore® Product Category: Concentration Kit Skip to the end of the images gallery Skip to the beginning of the images gallery Products The sample matrix is spiked with a known concentration of recombinant target protein. NA indicates the substance has not been tested for that assay. It provides ready-to-use convenience by supplying the dye reagent at 1x concentration and two protein assay standards at seven prediluted concentrations. Offering a fast and flexible approach to the assessment of protein concentration, characterization, and purity in a wide variety of samples. Process relevant information is recorded on “HSL_LAB_009. In response to these limitations, we have innovated a high-efficiency protein precipitation kit, a refinement of the traditional TCA method. 9 EU/mg). com/evportal/desti The Thermo Scientific Pierce Rapid Gold BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay optimized to measure (at 480 nm) total protein concentration compared to a. Using the qEV Concentration Kit, EVs can be concentrated to a customisable pellet, which can be used as it is for maximal EV enrichment, or resuspended in a desired volume and buffer type. For most determinations of protein concentration, relative values are generally sufficient. The concentration range of standards in the kits cover the linear range of the assay. Its absorbance Perform automated protein electrophoresis with the Bioanalyzer Protein analysis solution. Pierce™ BCA Protein Assay Kit The nine-point calibration curve was performed in duplicate from the BSA included in the kit in the concentration range from 0 to 2000 µg/mL. The assay has a dynamic range of 25 to 2,000 µg/ml and a lower limit detection (LLOD) of approximately 22 µg/ml. 01: BCA Data Capture Form. Product Description BCA Protein Quantification Kit is one of the most sensitive methods for measuring protein concentration. It is designed to assist users in reducing or eliminating the use of potassium cyanide (KCN) in their laboratory experiments. See how our XCELL PC System is changing the face of regenerative medicine. The concentration of pure antibodies also can be estimated using any commercial protein assay, such as our BCA Protein Assay Kit or Coomassie Plus Protein Assay Kit. The standard is used to prepare a series of solutions of known concentration by serial dilution of a protein stock solution followed by construction of a standard curve plotting concentration versus absorbance. Compounds are listed alphabetically under common names or abbreviations with the exception of sodium compounds, which are alphabetized under “Na”. Measure the total protein concentration. Introduction Typical protein assays are used to determine protein concentration by comparing the assay response of a sample to that of a standard whose concentration is known. If alternative buffers are to be used with the resin, we recommend prior assay optimization to any impact on the extant and/or specificity of protein binding. The assay supplies ready-to-use dye reagent at 1x concentration and two protein standards at seven prediluted concentrations (0. When mixed with a protein solution, the acidic coomassie-dye reagent changes color from brown to blue in proportion the amount of protein present in the sample. The kit employs an innovative separation material that is able to concentrate proteins and peptides while simultaneoulsy removing salts and other contaminants such as detergents and denaturants. Introduction The Qubit® Protein Assay Kits make protein quantitation easy and accurate. A simple Centrifuge the sample at 16,000 × g for 10 mins at 4°C. 2 days ago · The BCA Protein Assay Kit can be used to measure the protein concentration of lysates or homogenates, in microplate format, prepared with the following buffers: Cell Lysis Buffer (10X) #9803, RIPA Buffer (10X) #9806, PathScan® Sandwich ELISA Lysis Buffer (1X) #7018. The kit has been optimized for determining total protein content in low protein concentration samples (0. The InvitrogenTM QubitTM Protein BR Assay is a fluorometric assay that combines accuracy, compatibility, and ease of The Pierce Bradford Plus Protein Assay Kit is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent used to quickly measure total protein concentration compared to a protein standard. Western blot: Brain tissue lysates (10µL) were separated by SDS-PAGE, transferred to a nitrocellulose membrane and blocked for 1 hour. Protein concentration is determined both visually by comparing samples to a set of standards and by measuring absorbance on a SmartSpec™ spectrophotometer. Meridian Road Product Description: BCA Protein Assay Kit (Enhanced BCA Protein Assay Kit) is based on the BCA method, one of the two most commonly used protein concentration assays in the world, and realizes the simplicity, high stability, high sensitivity, and high compatibility of protein concentration determination. Features: Concentrate protein samples. 5). The Fine Bradford Protein Assay Kit has the characteristics of high sensitivity, light background and significant color difference, and the measured of protein in low concentration can be intuitive and convenient. 5mL, contains standardized BSA solutions at a specific concentration from 125 to 2000μg/mL in 0. Comparison to a standard curve provides a relative measurement of protein ELISA sample preparation guide Please refer to the protocol included with your kit for product-specific details regarding sample preparation and compatible sample types. Therefore, protein concentration can easily be determined within a few minutes in colorimetric detection. Figure 1. Flexible assay formats: Use the standard assay for protein concentrations between 200 and 1,400 µg/ml (20–140 µg total protein) Use the low-concentration assay for Kits can come with either pre-diluted protein standards of a listed concentration for a faster and easier use, or a lyophilized protein standard sample and heavily concentrated reagent for experimenters to manipulate and create their own concentration reagents and standards. Protein concentration is determined in one step, with no need to dilute standards The kits used for protein concentration determination were the Thermo Scientific BCA Protein Assay Kit and the Modified Lowry Protein Assay Kit (# 23225 and 23240). Plot a standard curve based on absorbance of the protein standards and determine the protein concentration of each unknown sample according to the Micro BCA Protein Assay Kit instructions. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of Achieve sensitive detection of protein, and low protein-to-protein variation, with the Qubit Protein and Protein BR Assay Kits for fluorescence-based protein quantitation assays. Protein identification and quantification have become extremely valuable in advancing our understanding and finding cures. The water-soluble complex has strong absorbance at A562 nm. The Protein (BCA) assay kit is an easy and fast way to quantify protein concentration in your sample. To run one immunoassay and one Total Protein assay - Select Total Protein, then select any combination of chemiluminescence or fluorescence for Probe 1. BSA is the most commonly used standard for relative protein concentration determination in most laboratories Abcam's BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. C6667). This Micro Lowry Protein Assay Kit (Peterson modification) is used to assay protein concentrations, with adaptations from the Lowry method for more general use. ProteoSpin™ Urine Protein Concentration Midi Kit The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Ideal for mass spectrometry, Western blot, PCR, and RNA sequencing methods. For example, in the presence of Mg2+, Ca2+ or Zn2+, DNase I degrades DNA by making random single-strand nicks in the phosphate backbone. The wide spectral separation of this dye from commonly used red Background The Thermo Scientific Pierce 660 nm Protein Assay is a quick, ready-to-use colorimetric method for total protein quantitation. Based on the accuracy required and the amount and purity of the protein available, different methods are suitable for determining protein concentration. Purification is based on spin column chromatography using Antibody purification using the Amicon® Pro Affinity Concentration Protein A Kit consists of three basic steps (binding, wash, and elution) as well as sample concentration and buffer exchange. It gradually lyses cells, separates the intact nuclei from the Protein concentrations are estimated by reference to absorbances obtained for a series of standard protein dilutions, which are assayed alongside the unknown samples. Molecular Probes’ Alexa Fluor®647 Protein Labeling Kit provides a convenient means to label proteins with our superior Alexa Fluor 647 dye. The Thermo Scientific Pierce Modified Lowry Protein Assay is a stable form of a traditional, 2-component, folin phenol- and copper-based reagent system to measure (A750nm) total protein concentration. 25 to 50 mg/ml and is detergent compatible. The latter is simple and convenient to operate, requiring only a small amount (10-25 μL) of protein sample The color response curves for the Modified Lowry Protein Assay and the original Lowry method have nearly 100% correlation. Dilute samples to 10 mg protein/mL with 1X PBS. It provides an ultra-sensitive method to detect and quantify total protein concentration. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. Let us consider a second case. Overview The BCA Protein Assay Kit employs a modification of the bicinchoninic acid (BCA) method (also known as the Smith Assay) for the colorimetric detection of protein concentration or total protein determination in a sample. 75, 1. By spinning, excess solution elutes out, reducing the final volume of the sample while retaining the particles of Assay Standards Bio-Rad kits include either of two different proteins, BSA or BGG, as standards to construct a standard curve for the relative quantitation of the proteins in the samples. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. Kit components are sufficient to complete500 standard-size reactions (50 µl proteinsample plus 1 ml reagent) or 2500 micro-scalereactions (25 µl protein sample plus 200 µlreagent). Optimum sample preparation procedures will vary depending on the target and assay of interest. , Thermo Scientific™ BCA Protein Assay Kit, P/N 23227) C. Protein Assay Kits A variety of protein assay and quantification kits are available for the rapid and reliable measurement of total protein abundance or concentration. Procedure 1. Learn the basics of protein assay data analysis and developing a standard curve for determining protein concentration. Urine protein analysis can be used for the identification of potential biomarkers in urine, and to diagnose and/or monitor renal and other diseases. Upon binding to proteins, it forms strong, noncovalent complexes and Nov 1, 2020 · In Eq. Product description The Thermo ScientificTM PierceTM BCA Protein Assay Kit with Dilution-FreeTM BSA Protein Standards is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. 0, 1. 5-40 μg/mL), even in the presence of detergents. ufm8a0 cjq6e uc w2 zyx t8cevqc i9f anqhn xvhs scntc